A histochemical study of lactic dehydrogenase in Paget cells.

By the use of a modified incubating medium, lactic dehydrogenase in Paget cells was histochemically examined. The addition of phenazine methosulfate to the substrate solution decreased the reaction intensity of lactic dehydrogenase in Paget cells. In the presence of potassium cyanide, however, phenazine methosulfate caused increased reaction. When urea was introduced into the substrate solution, the lactic dehydrogenase reaction of malpighian cells was strikingly decreased, while the reaction of Paget cells was not decreased so much. The reaction in the sections, which were incubated in the excess lactate-containing solution, was slightly decreased in these two cells. On the basis of biological and biochemical considerations, these facts appear to indicate that there was a metabolic difference between normal malpighian cells and Paget cells.